Part:BBa_M33356:Design
L28H-fnr CDS
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 777 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 778
Illegal PstI site found at 792
Illegal NotI site found at 7
Illegal NotI site found at 785 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 778 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 778
Illegal PstI site found at 792 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
No RBS (ribosome binding sites) were included in the detailed design of the sequence. This is because it would constrain the user of this piece to only one RBS when this fnr* gene would be used. Also, the addition of the TAATAA at the end of the CDS at this piece would force a stop to the translation.
Source
This part was directly copied from a genomic sequence--specifically from the EcoGene DNA database (ecogene.org). The selection was from 111 bp upstream of fnr CDS (coding sequence), to the end of the CDS. Afterward, the following edits were made: the last ten base-pairs before the CDS were deleted as they were part of the RBS (ribosome binding site) of the fnr (Shaw, Guest 1982). Also, the required Leucine to Histidine switch was made in the CDS. Finally, the sequence TAATAA replaced the last three nucleotides of the coding sequence.